Isolation of Microorganisms Through Air Exposure and Human Skin Swabbing and Testing of Alcogel Effectiveness in Eliminating Microorganisms
Autor: Maryam • November 23, 2017 • 2,276 Words (10 Pages) • 792 Views
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A half strength Nutrient Agar medium and half strength Potato Dextrose Agar medium were then exposed inside the women’s comfort room for one to two minutes. Other groups exposed their media in the cafeteria, autoclave room, men’s comfort room, ATM, faculty, lockers and others. Another set of half strength Nutrient Agar medium and half strength Potato Dextrose Agar medium were exposed to human skin through cotton swab method. All the samples were then place inside the locker for at room temperature for 24 hours.
The remaining set of half strengths Nutrient Agar and Potato Dextrose Agar media were then put in direct contact with human thumbs over the mediums. The right thumb was applied with alcogel and the left with the absence of alcogel in each half strength Nutrient Agar and strength Potato Dextrose Agar medium. And incubated inside the locker for 24 hours at room temperature.
As 24 hours passed by then the plates were checked whether colonies where formed and were counted.
Results
Table No. 1: Results for Isolating Airborne Microorganisms through Air Exposure
Group
Number
Sites where Air Exposure was done
Half Strength Nutrient Agar Plate
Half Strength Potato Dextrose Agar Plate
1
Female’s Comfort Room
4
0
2
Cafeteria
2
2
3
General Education Faculty Room
4
1
4
ATM
21
1
5
Stairs
11
12
6
Men’s Comfort Room
0
0
7
Autoclave Room
1
2
8
Locker
7
2
9
Carpark
3
4
Table No. 2: Results for Isolating Microorganisms in the Human Skin through Swabbing
Group
Number
Body Part
Half Strength Nutrient Agar Plate
Half Strength Potato Dextrose Agar Plate
1
Face (Pimple)
0
0
2
Ears
21
9
3
In between fingers
61
24
4
Sole of the feet
TNTC
10
5
Behind the knees
22
0
6
Under the tongue
0
0
7
Bikini area
134
66
8
Belly button
TNTC
TNTC
9
Nose
TNTC
TNTC
Table No. 3: Results for Testing of the Effectiveness of Alcogel on Eliminating Microorganisms
Group
Number
Alcogel
Brand
Half Strength Nutrient Agar Plate
Half Strength Potato Dextrose Agar Plate
With Alcogel
Without Alcogel
With Alcogel
Without Alcogel
1
Hygienex
41
10
23
1
2
Bench
22
10
25
0
3
Alcodex
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