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Isolation of Microorganisms Through Air Exposure and Human Skin Swabbing and Testing of Alcogel Effectiveness in Eliminating Microorganisms

Autor:   •  November 23, 2017  •  2,276 Words (10 Pages)  •  620 Views

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A half strength Nutrient Agar medium and half strength Potato Dextrose Agar medium were then exposed inside the women’s comfort room for one to two minutes. Other groups exposed their media in the cafeteria, autoclave room, men’s comfort room, ATM, faculty, lockers and others. Another set of half strength Nutrient Agar medium and half strength Potato Dextrose Agar medium were exposed to human skin through cotton swab method. All the samples were then place inside the locker for at room temperature for 24 hours.

The remaining set of half strengths Nutrient Agar and Potato Dextrose Agar media were then put in direct contact with human thumbs over the mediums. The right thumb was applied with alcogel and the left with the absence of alcogel in each half strength Nutrient Agar and strength Potato Dextrose Agar medium. And incubated inside the locker for 24 hours at room temperature.

As 24 hours passed by then the plates were checked whether colonies where formed and were counted.

Results

Table No. 1: Results for Isolating Airborne Microorganisms through Air Exposure

Group

Number

Sites where Air Exposure was done

Half Strength Nutrient Agar Plate

Half Strength Potato Dextrose Agar Plate

1

Female’s Comfort Room

4

0

2

Cafeteria

2

2

3

General Education Faculty Room

4

1

4

ATM

21

1

5

Stairs

11

12

6

Men’s Comfort Room

0

0

7

Autoclave Room

1

2

8

Locker

7

2

9

Carpark

3

4

Table No. 2: Results for Isolating Microorganisms in the Human Skin through Swabbing

Group

Number

Body Part

Half Strength Nutrient Agar Plate

Half Strength Potato Dextrose Agar Plate

1

Face (Pimple)

0

0

2

Ears

21

9

3

In between fingers

61

24

4

Sole of the feet

TNTC

10

5

Behind the knees

22

0

6

Under the tongue

0

0

7

Bikini area

134

66

8

Belly button

TNTC

TNTC

9

Nose

TNTC

TNTC

Table No. 3: Results for Testing of the Effectiveness of Alcogel on Eliminating Microorganisms

Group

Number

Alcogel

Brand

Half Strength Nutrient Agar Plate

Half Strength Potato Dextrose Agar Plate

With Alcogel

Without Alcogel

With Alcogel

Without Alcogel

1

Hygienex

41

10

23

1

2

Bench

22

10

25

0

3

Alcodex

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