Virtual Nitrogen Balance Practical
Autor: Mikki • October 25, 2017 • 1,082 Words (5 Pages) • 806 Views
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Sample – high (ml)
1.0
1.0
Sample – medium (ml)
1.0
1.0
Sample – low (ml)
1.0
1.0
Sample – X (ml)
1.0
1.0
Sample –Y (ml)
1.0
1.0
Sample – Z (ml)
1.0
1.0
Alkaline picrate (ml)
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
Distilled water (ml)
7.0
7.0
7.0
7.0
7.0
7.0
7.0
7.0
7.0
7.0
7.0
7.0
Absorbance 520 nm
0.328
0.318
0.325
0.323
0.318
0.318
0.350
0.346
0.278
0.272
0.273
0.261
Creatinine (⎧g)
[pic 2]
Line of best fit: y = 0.006x + 0.0111
Where y= absorption (520nm) and x= creatinine (µg)
Sample
Absorbance
µg/1ml
Average µg/1ml
Undiluted µg/1ml
X 20
mg/ml
/1000
Mg/24hr (2L)
X 2000
g/24hr
/ 1000
Nitrogen g/24hr
X 0.371
High
0.328
52.8
52
1040
1.04
2080
2.08
0.77
0.318
51.2
Med
0.325
52.3
52.2
1044
1.04
2088
2.08
0.77
0.323
52.0
Low
0.318
51.2
51.2
1024
1.024
2048
2.05
0.76
0.318
51.2
X
0.350
56.5
56.15
1123
1.123
2246
2.246
0.83
0.346
55.8
Y
0.278
44.5
44.0
880
0.88
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